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chemically competent e. coli bacteria one shot top10  (Thermo Fisher)


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    Structured Review

    Thermo Fisher chemically competent e. coli bacteria one shot top10
    Chemically Competent E. Coli Bacteria One Shot Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e%2E+coli+one+shot+top10/e++coli+top10/pm40410189-327-7-11
    Average 90 stars, based on 1 article reviews
    chemically competent e. coli bacteria one shot top10 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Transformation Assay:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Virus:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Derivative Assay:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Recombinant:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Polymerase Chain Reaction:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Clone Assay:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Purification:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Titration:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Fluorescence:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Competitive Binding Assay:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Concentration Assay:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Modification:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Expressing:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Solubility:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Centrifugation:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Control:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Staining:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Standard Deviation:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Construct:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Electroporation:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.

    Transduction:

    Article Title: Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum
    Article Snippet: U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ- (Thermo Fisher Scientific, Langerwehe, Germany) E. coli One Shot TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG (Invitrogen - Thermo Fisher Scientific, Langerwehe, Germany) E. coli BL21(DE3) F– ompT gal dcm lon hsdSB(rB–mB– ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) (Thermo Fisher Scientific, Langerwehe, Germany) C. glutamicum ATCC13032 K9 htrA::htrA ́-eyfp (replacement of htrA by htrA ́-eyfp); biosensor strain used for secretion experiments. (Jurischka et al., 2020)

    Article Title: SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1
    Article Snippet: These plasmids were constructed and cloned in E. coli One Shot TOP10 (Thermo) and subsequently transformed into S. aureus strain RN4220 by electroporation [ ] before transduction into appropriate S. aureus strains using phage ɸ11.

    Article Title: Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi ( Plukenetia volubilis )
    Article Snippet: This mixture was ligated into the PCR 2.1 vector and cloned using chemically competent cells of E. coli One Shot TOP10 (Invitrogen, Carlsbad, CA, USA) by heat shock.

    Article Title: Protective role of extracellular vesicles against oxidative DNA damage.
    Article Snippet: To obtain the necessary amount of plasmid, it was transformed into a commercial bacterial system (E. coli One Shot TOP10, Thermo Fisher Scientific, Whaltham, MA, USA), which was allowed to grow.

    Article Title: Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan
    Article Snippet: Amplicons were cloned into pCR 2.1-TOPO (Invitrogen) and further transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: In Vitro Evolution of Listeria monocytogenes Reveals Selective Pressure for Loss of SigB and AgrA Function at Different Incubation Temperatures.
    Article Snippet: The digested DNA was ligated using T4 ligase (Promega) and subsequently transformed into E. coli One Shot TOP10 (Invitrogen).

    Article Title: TFEB triggers a matrix degradation and invasion program in triple-negative breast cancer cells upon mTORC1 repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey Anti-Mouse IgG Antibody, AF488 Molecular Probes Cat#A21202; RRID: AB_141607 Cy3-AffiniPure Donkey Anti-Mouse IgG Jackson ImmunoResearch Labs Cat#715-165-151; RRID: AB_2315777 Donkey Anti-Mouse IgG Polyclonal Antibody Molecular Probes Cat#A31571; RRID: AB_162542 Donkey Anti-Rabbit IgG Antibody, AF488 Molecular Probes Cat#A-21206; RRID: AB_2535792 Cy3-AffiniPure Donkey Anti-Rabbit IgG Jackson ImmunoResearch Labs Cat#711-165-152; RRID: AB_2307443 Bacterial and virus strains E. coli One Shot TOP10 [F- mcrA D( mrr-hsdRMSmcrBC) F80lacZDM15 D lacX74 recA1 araD139 D( araleu) 7697 galU galK rpsL (StrR) endA1 nupG] Invitrogen Cat#C404010 Biological samples Patients Derived Xenografts (PDXs) Preclinical Investigation Laboratory, Translational Research Department, Institut Curie (Paris, France) https://institut-curie.org/plateforme/ curiecoretech-vivo-preclinicalexperiments Chemicals, peptides, and recombinant proteins Apilimod Euromedex Cat#S6414 Bafilomycin A1 Sigma Cat#196000 Doxorubicin (Doxo) Cell Signaling TECHNOLOGY Cat#5927 Eltrombopag (ElO) Sigma Cat#SML3182 Everolimus (Evo) Novartis Pharma SAS Cat#AFINITOR 10 mg Cpr Plq/30 GM6001 Merck Millipore Cat#CC1100 Rapamycin (Rapa) Tocris Biotechne Cat#1292 Torin-1 Tocris Biotechne Cat#4247 Phalloidin AF488 Molecular Probes Cat#A12379 Phalloidin AF546 Molecular Probes Cat#A22283 Phalloidin AF633 Molecular Probes Cat#A22284 Deposited data Raw and analyzed RNA-Seq data This paper GEO: GSE242138 Experimental models: Cell lines

    Article Title: Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression
    Article Snippet: E. coli One Shot TOP10 (Invitrogen, Thermo Fisher Scientific, Waltham, USA) cells were used for the propagation of all plasmids.



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